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Image Search Results
Journal: Cell
Article Title: A membrane-associated MHC-I inhibitory axis for cancer immune evasion
doi: 10.1016/j.cell.2023.07.016
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: For intracellular staining, cells were permeabilized with 0.1% saponin in PBS + 2% BSA containing Fc blocker for 30 min at room temperature, then stained overnight at 4 °C with anti-mouse EEA1 antibody (Thermo Fisher Scientific # MA5–31575, dilution 1:100), or APC anti-mouse CD107a (LAMP1) (Miltenyi #130–111-505, dilution 1:50), or
Techniques: Control, Purification, Blocking Assay, Virus, Recombinant, Protease Inhibitor, Transfection, Immunoprecipitation, Activation Assay, Magnetic Beads, Reverse Transcription, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Double Knockout, shRNA, Real-time Polymerase Chain Reaction, Genome Wide, Plasmid Preparation, Software, Flow Cytometry